Nucleora turns reference genomes into an interactive workbench: search a real antigen, codon-optimize it for the animal you're targeting, assemble a complete mRNA construct, fold the RNA, simulate the manufacturing run, and price out the order — all in one local-first window, with 530+ tools underneath when you need them.
Free while in development · macOS & Windows · your sequences stay on your computer
No stitching together five web tools and a spreadsheet. Nucleora keeps the design, the analysis, and the numbers in the same place — real BioPython, real ViennaRNA folding, real codon-usage tables, computed on your machine.
Re-code a protein for any host — including elephants, okapi, and other conservation species with real or clade-proxy codon tables — with a live before/after readout.
Assemble T7 promoter, 5′UTR, Kozak, signal peptide, antigen ORF, 3′UTR and poly(A) into a therapeutic-style mRNA, with every part's provenance cited.
Fold with the ViennaRNA engine to see secondary structure, minimum free energy, and dsRNA/hairpin immunogenicity risk — computed locally.
Walk the construct through an in-vitro-transcription run, get a graded manufacturability report, then estimate yield, kinetics, and an itemized order form.
Restriction mapping, Gibson and Golden Gate assembly, and primer design via primer3 — with a built-in fallback where primer3 has no prebuilt wheel.
Virtual agarose gels, in-silico PCR, peptide property calculators, and pairwise/multiple alignment — the everyday bench assays, simulated.
Codon choice has to match the animal being dosed. Nucleora ships real codon tables built from NCBI RefSeq coding sequences for African elephant, Asian elephant, and cattle — and where a species like okapi or bongo has almost no sequenced genes, it optimizes using a documented, clade-anchored proxy instead of pretending the data exists.
A real walkthrough of the Nucleora interface — genuine ViennaRNA folding, genuine codon optimization, genuine manufacturability scoring. No mockups.
Watch the demo